KEY TERMS:
- A parental strand or duplex of DNA refers to the DNA that will be replicated.
- The antisense strand (Template strand) of DNA is complementary to the sense strand, and is the one that acts as the template for synthesis of mRNA.
- A daughter strand or duplex of DNA refers to the newly synthesized DNA.
- Semiconservative replication is accomplished by separation of the strands of a parental duplex, each then acting as a template for synthesis of a complementary strand.
KEY CONCEPTS:
- The Meselson-Stahl experiment used density labeling to prove that the single polynucleotide strand is the unit of DNA that is conserved during replication.
- Each strand of a DNA duplex acts as a template to synthesize a daughter strand.
- The sequences of the daughter strands are determined by complementary base pairing with the separated parental strands.
It is crucial that the genetic material is reproduced
accurately. Because the two polynucleotide strands are joined only by hydrogen
bonds, they are able to separate without requiring breakage of covalent bonds.
The specificity of base pairing suggests that each of the separated parental strands could act as a template strand for the synthesis of a complementary
daughter strand. Figure 1.11
shows the principle that a new daughter strand is assembled on each parental
strand. The sequence of the daughter strand is dictated by the parental strand;
an A in the parental strand causes a T to be placed in the
daughter strand, a parental G directs incorporation of a daughter
C, and so on.
The top part of the figure shows a parental (unreplicated)
duplex that consists of the original two parental strands. The lower part shows
the two daughter duplexes that are being produced by complementary base pairing.
Each of the daughter duplexes is identical in sequence with the original parent,
and contains one parental strand and one newly synthesized strand. The
structure of DNA carries the information needed to perpetuate its sequence.
The consequences of this mode of replication are illustrated
in Figure 1.12. The parental duplex is replicated to form
two daughter duplexes, each of which consists of one parental strand and one
(newly synthesized) daughter strand. The unit conserved from one generation
to the next is one of the two individual strands comprising the parental
duplex. This behavior is called semiconservative
replication.
The figure illustrates a prediction of this model. If the
parental DNA carries a "heavy" density label because the organism has been grown
in medium containing a suitable isotope (such as 15N), its strands
can be distinguished from those that are synthesized when the organism is
transferred to a medium containing normal "light" isotopes.
The parental DNA consists of a duplex of two heavy strands
(red). After one generation of growth in light medium, the duplex DNA is
"hybrid" in density—it consists of one heavy
parental strand (red) and one light daughter strand (blue). After a second
generation, the two strands of each hybrid duplex have separated; each gains a
light partner, so that now half of the duplex DNA remains hybrid while half is
entirely light (both strands are blue).
The individual strands of these duplexes are entirely
heavy or entirely light. This pattern was confirmed experimentally in the
Meselson-Stahl experiment of 1958, which followed the semiconservative
replication of DNA through three generations of growth of E. coli. When
DNA was extracted from bacteria and its density measured by centrifugation, the
DNA formed bands corresponding to its density—heavy for parental, hybrid for the first generation,
and half hybrid and half light in the second generation (Meselson and Stahl,
1958; for review see Holmes, 2001).
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