KEY CONCEPTS:
- Phage infection proved that DNA is the genetic material of viruses. When the DNA and protein components of bacteriophages are labeled with different radioactive isotopes, only the DNA is transmitted to the progeny phages produced by infecting bacteria.
Figure 1.5 illustrates the results of
an experiment in 1952 in which bacteria were infected with T2 phages that had
been radioactively labeled either in their DNA component (with
32P) or in their protein component (with 35S).
The infected bacteria were agitated in a blender, and two fractions were
separated by centrifugation. One contained the empty phage coats that were
released from the surface of the bacteria. The other fraction consisted of the
infected bacteria themselves.
Most of the 32P label was present in the infected
bacteria. The progeny phage particles produced by the infection contained ~30%
of the original 32P label. The progeny received very little—less than 1%—of the
protein contained in the original phage population. The phage coats consist of
protein and therefore carried the 35S radioactive label. This
experiment therefore showed directly that only the DNA of the parent phages
enters the bacteria and then becomes part of the progeny phages, exactly the
pattern of inheritance expected of genetic material (Hershey and Chase, 1952).
A phage (virus) reproduces by commandeering the machinery of
an infected host cell to manufacture more copies of itself. The phage possesses
genetic material whose behavior is analogous to that of cellular genomes: its
traits are faithfully reproduced, and they are subject to the same rules that
govern inheritance. The case of T2 reinforces the general conclusion that the
genetic material is DNA, whether part of the genome of a cell or virus.
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